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KMID : 0869020070100020065
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Journal of Korean Orthopaedic Research Society
2007 Volume.10 No. 2 p.65 ~ p.75
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Cross-talk between Integrin and SMAD Signal Pathway in Articular Chondrocyte
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Hahn Soo-Bong
Park Min-Sung
Kim Yun-Hee
Lee Jin-Woo
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Abstract
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Purpose: Identifying the signal cross-talk between integrin signaling cascade and TGF-beta 1 signaling cascade in articular chondrocytes.
Materials and Methods: To analyze integrin or TGF-beta 1 mediated signaling pathways from extracellular stimuli, type II collagen was coated on the cell culture plate and TGF-beta 1 was added to cell culture media. Chondrocytes were cultured in the conditioned media with each or both stimuli. Altered activation of signaling proteins detected with western blot technique.
Results: More rapid attachment of cells was observed in the type II collagen coated group than non-coated group. The phosphorylated SMAD 2 and 3 were expressed in the type II collagen coated group and synergistically up-regulated phosphorylation in the co-treated group. The phosphorylated FAK at tyrosine 925 was activated by TGF-beta 1 treatment and synergistically up-regulated by both stimuli. But there was no meaningfully changed phosphorylation of extracellular signal regulated protein kinase (ERK) 1/2 and p38, as known downstream molecules of FAK cascade.
Conclusion: This result means that SMAD 2, SMAD 3 and tyrosine 925 of FAK are involved in this signal cross-talking in articular chondrocytes.
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KEYWORD
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Articular chondrocyte, Signal cross-talk, Type II colagen, TGF-β1, FAK, SMAD 2/3
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